Target search by an imported conjugative DNA element for a unique integration site along a bacterial chromosome during horizontal gene transfer.

ABSTRACT: Integrative and conjugative elements (ICEs) are mobile genetic elements that can transfer by conjugation to recipient cells. Some ICEs integrate into a unique site in the genome of their hosts. We studied quantitatively the process by which an ICE searches for its unique integration site in the Bacillus subtilis chromosome. We followed the motion of both ICEBs1 and the chromosomal integration site in real time within individual cells. ICEBs1 exhibited a wide spectrum of dynamical behaviors, ranging from rapid sub-diffusive displacements crisscrossing the cell, to kinetically trapped states. The chromosomal integration site moved sub-diffusively and exhibited pronounced dynamical asymmetry between longitudinal and transversal motions, highlighting the role of chromosomal structure and the h

SUBMITTER: Arbel-Goren R 

PROVIDER: S-EPMC10123120 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature