PDCL2 is essential for sperm acrosome formation and male fertility in mice.

ABSTRACT:

Background

Each year, infertility affects 15% of couples worldwide, with 50% of cases attributed to men. Globozoospermia is an uncommon cause of male factor infertility, characterized by defects in sperm acrosome formation, leading to round-headed spermatozoa.

Objective

We generated Pdcl2 knockout mice to investigate the essential roles of PDCL2 in mammalian reproduction.

Materials and methods

We used reverse transcription-polymerase chain reaction to demonstrate that PDCL2 was expressed exclusively in the male reproductive tract in mice and humans. We created Pdcl2 knockout mice using the CRISPR-Cas9 system and analyzed their fertility. Pdcl2 null spermatozoa underwent further evaluation using computer-assisted sperm analysis, light microscopy, and ultrastructural mi

SUBMITTER: Fujihara Y 

PROVIDER: S-EPMC10123174 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature