A highly sensitive colorimetric approach based on tris (bipyridine) Ruthenium (II/III) mediator for the enzymatic detection of phenylalanine.

ABSTRACT: The accurate monitoring of phenylalanine concentration plays a prominent role in the treatment of phenylketonuria (PKU). In this study, we present an enzymatic assay based on Phenylalanine Dehydrogenase/NAD⁺ and tris (bipyridine) Ruthenium (II/III) as a colorimetric mediator for the detection of Phenylalanine concentration. The amount of amino acid was quantitatively recognized by optical absorption measurements at 452 nm through the conversion of Ru (byp)₃ ³⁺ to Ru (byp)₃ ²⁺, which is induced by the neoformed NADH. A detection limit of 0.33 µM, a limit of quantification of 1.01 µM, and a sensitivity of 36.6 a.u nM^-1 were obtained. The proposed method was successfully tested using biological specimens from patients affected

SUBMITTER: Messina MA 

PROVIDER: S-EPMC10123266 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature