Glucose determination in human serum by applying inner filter effect quenching mechanism of upconversion nanoparticles.

ABSTRACT: Accurate blood glucose determination is essential to the clinical diagnosis and management of diabetes. This work establishes an inner filter effect (IFE) strategy between upconversion nanoparticles (UCNPs) and quinone-imine complex for glucose monitoring in human serum simply and efficiently. In this system, the enzyme glucose oxidase (GOx) catalyzes the reaction of glucose into hydrogen peroxide (H₂O₂) and gluconic acid when compulsion by oxygen. In the presence of horseradish peroxidase (HRP), the produced H₂O₂ can catalytically oxidize phenol and 4-amino antipyrine (4-AAP) to generate quinone-imine products. The purple-colored quinone-imine complex effectively absorbed the fluorescence of NaYF₄:Yb³⁺, Er³⁺ UCNP

SUBMITTER: Chen X 

PROVIDER: S-EPMC10123268 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature