Protocol for in vitro fluorescence assay of papain-like protease and cell-based immunofluorescence assay of coronavirus infection.
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SUBMITTER: Chang J
PROVIDER: S-EPMC10123355 | biostudies-literature | 2023 Apr
REPOSITORIES: biostudies-literature
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Protocol for in vitro fluorescence assay of papain-like protease and cell-based immunofluorescence assay of coronavirus infection.
Chang JuOae J Kim Jinhee J Hong Subin S Jeong Kwiwan K Kim Seungtaek S Lee Wonsik W
STAR protocols 20230424 2
Here, we describe detailed steps to constitute an in vitro assay for monitoring papain-like protease of coronavirus and a cell-based immunofluorescence infection assay. These assays can be adapted for high-throughput screening to determine the efficacy of novel protease inhibitors of coronaviruses and other viruses. In addition, cell-based immunofluorescence infection assay can be used to visually analyze antiviral efficacy of any novel compounds. For complete details on the use and execution of ...[more]
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