Comparison of Pronase versus Manual Dechorionation of Zebrafish Embryos for Small Molecule Treatments.

ABSTRACT: Zebrafish are a powerful animal model for small molecule screening. Small molecule treatments of zebrafish embryos usually require that the chorion, an acellular envelope enclosing the embryo, is removed in order for chemical compounds to access the embryo from the bath medium. For large-scale studies requiring hundreds of embryos, manual dechorionation, using forceps, can be a time-consuming and limiting process. Pronase is a non-specific protease that is widely used as an enzymatic alternative for dechorionating zebrafish embryos. However, whether pronase treatments alter the effects of subsequent small molecule treatments has not been addressed. Here, we provide a detailed protocol for large-scale pronase dechorionation of zebrafish embryos. We tested whether pronase treatment can influ

SUBMITTER: Hasegawa EH 

PROVIDER: S-EPMC10123619 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature