CellComm infers cellular crosstalk that drives haematopoietic stem and progenitor cell development.

ABSTRACT: Intercellular communication orchestrates a multitude of physiologic and pathologic conditions. Algorithms to infer cell-cell communication and predict downstream signalling and regulatory networks are needed to illuminate mechanisms of stem cell differentiation and tissue development. Here, to fill this gap, we developed and applied CellComm to investigate how the aorta-gonad-mesonephros microenvironment dictates haematopoietic stem and progenitor cell emergence. We identified key microenvironmental signals and transcriptional networks that regulate haematopoietic development, including Stat3, Nr0b2, Ybx1 and App, and confirmed their roles using zebrafish, mouse and human models. Notably, CellComm revealed extensive crosstalk among signalling pathways and convergence on common transcriptio

SUBMITTER: Lummertz da Rocha E 

PROVIDER: S-EPMC10123873 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature