Optimized Repli-seq: improved DNA replication timing analysis by next-generation sequencing.

ABSTRACT: The human genome is divided into functional units that replicate at specific times during S-phase. This temporal program is known as replication timing (RT) and is coordinated with the spatial organization of the genome and transcriptional activity. RT is also cell type-specific, dynamically regulated during development, and alterations in RT are observed in multiple diseases. Thus, the precise measure of RT is critical to understand the role of RT in gene function regulation. Distinct methods for assaying the RT program exist; however, conventional methods require thousands of cells as input, prohibiting its applicability to samples with limited cell numbers such as those from disease patients or from early developing embryos. Although single-cell RT analyses have been developed, these me

SUBMITTER: Rivera-Mulia JC 

PROVIDER: S-EPMC10124313 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature