Tumor and immune remodeling following radiotherapy in human renal cell carcinoma.

ABSTRACT:

Background

Studies evaluating peripheral patient samples show radiation can modulate immune responses, yet the biological changes in human tumors particularly at the cellular level remain largely unknown. Here, we address how radiation treatment shapes the immune compartment and interactions with cancer cells within renal cell carcinoma (RCC) patient tumors.

Methods

To identify how radiation shaped the immune compartment and potential immune interactions with tumor cells we evaluated RCC tumors from patients treated only with nephrectomy or with radiation followed by nephrectomy. Spectral flow cytometry using a 35-marker panel was performed on cell suspensions to evaluate protein expression within immune subsets. To reveal how radiation alters programming of immune populatio

SUBMITTER: Chow J 

PROVIDER: S-EPMC10124322 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature