Metabolic shift and the effect of mitochondrial respiration on the osteogenic differentiation of dental pulp stem cells.

ABSTRACT:

Background

Metabolism shifts from glycolysis to mitochondrial oxidative phosphorylation are vital during the differentiation of stem cells. Mitochondria have a direct function in differentiation. However, the metabolic shift and the effect of mitochondria in regulating the osteogenic differentiation of human dental pulp stem cells (hDPSCs) remain unclear.

Methods

Human dental pulp stem cells were collected from five healthy donors. Osteogenic differentiation was induced by osteogenic induction medium. The activities of alkaline phosphatase, hexokinase, pyruvate kinase, and lactate dehydrogenase were analyzed by enzymatic activity kits. The extracellular acidification rate and the mitochondrial oxygen consumption rate were measured. The mRNA levels of COL-1, ALP, TFAM,

SUBMITTER: Wan L 

PROVIDER: S-EPMC10124543 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature