NMR structure of emfourin, a novel protein metalloprotease inhibitor: Insights into the mechanism of action.

ABSTRACT: Emfourin (M4in) is a protein metalloprotease inhibitor recently discovered in the bacterium Serratia proteamaculans and the prototype of a new family of protein protease inhibitors with an unknown mechanism of action. Protealysin-like proteases (PLPs) of the thermolysin family are natural targets of emfourin-like inhibitors widespread in bacteria and known in archaea. The available data indicate the involvement of PLPs in interbacterial interaction as well as bacterial interaction with other organisms and likely in pathogenesis. Arguably, emfourin-like inhibitors participate in the regulation of bacterial pathogenesis by controlling PLP activity. Here, we determined the 3D structure of M4in using solution NMR spectroscopy. The obtained structure demonstrated no significant similarity to kn

SUBMITTER: Bozin TN 

PROVIDER: S-EPMC10124921 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature