Metabolomics Differences of the Donor Livers Between In Situ and Ex Situ Conditions During Ischemia-free Liver Transplantation.

ABSTRACT:

Background

Ischemia-free liver transplantation (IFLT) has been innovated to avoid graft ischemia during organ procurement, preservation, and implantation. However, the metabolism activity of the donor livers between in the in situ and ex situ normothermic machine perfusion (NMP) conditions, and between standard criteria donor and extend criteria donor remains unknown.

Methods

During IFLT, plasma samples were collected both at the portal vein and hepatic vein of the donor livers in situ during procurement and ex situ during NMP. An ultra-high performance liquid chromatography-mass spectrometry was conducted to investigate the common and distinct intraliver metabolite exchange.

Results

Profound cysteine and methionine metabolism, and aminoacyl-tRNA biosynthesis were fou

SUBMITTER: Guo Z 

PROVIDER: S-EPMC10125122 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature