A massively parallel screening platform for converting aptamers into molecular switches.

ABSTRACT: Aptamer-based molecular switches that undergo a binding-induced conformational change have proven valuable for a wide range of applications, such as imaging metabolites in cells, targeted drug delivery, and real-time detection of biomolecules. Since conventional aptamer selection methods do not typically produce aptamers with inherent structure-switching functionality, the aptamers must be converted to molecular switches in a post-selection process. Efforts to engineer such aptamer switches often use rational design approaches based on in silico secondary structure predictions. Unfortunately, existing software cannot accurately model three-dimensional oligonucleotide structures or non-canonical base-pairing, limiting the ability to identify appropriate sequence elements for targeted modifi

SUBMITTER: Yoshikawa AM 

PROVIDER: S-EPMC10126150 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature