Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour.

ABSTRACT:

Background

The transition of the myometrium from a quiescent to a contractile state during labour is known to involve inflammation, which is characterized by the infiltration of immune cells and the secretion of cytokines. However, the specific cellular mechanisms underlying inflammation in the myometrium during human parturition are not yet fully understood.

Methods

Through the analysis of transcriptomics, proteomics, and cytokine arrays, the inflammation in the human myometrium during labour was revealed. By performing single-cell RNA sequencing (scRNA-seq) and spatiotemporal transcriptomic (ST) analyses on human myometrium in term in labour (TIL) and term in non-labour (TNL), we established a comprehensive landscape of immune cells, their transcriptional characteristics,

SUBMITTER: Ji K 

PROVIDER: S-EPMC10126311 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature