Dataset Information

SH3BP1 Regulates Melanoma Progression Through Race1/Wace2 Signaling Pathway.

ABSTRACT:

Background

SH3-domain binding protein-1 (SH3BP1), which specifically inactivates Rac1 and its target protein Wave2, has been shown to be an important regulator of cancer metastasis. However, the effects of SH3BP1 in melanoma progression remain unclear. The current study aimed to explore the function of SH3BP1 in melanoma and its possible molecular mechanism.

Methods

TCGA database was used to analyze the expression of SH3BP1 in melanoma. Then, reverse transcription-quantitative polymerase chain reaction was performed to detect the expression of SH3BP1 in melanoma tissues and cells. Next, genes related to SH3BP1 were analyzed by LinkedOmics database, and protein interactions were analyzed by STRING database. These genes were further subjected to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis. In addition, the signaling pathway of SH3BP1 action was screened by bioinformatics analysis. Finally, the function of SH3BP1 and its mediated signaling pathway in melanoma progression were investigated in vitro and in vivo.

Results

SH3BP1 was significantly upregulated in melanoma tissues and cells. The pathways regulated by SH3BP1 are closely related to the occurrence and development of tumors. And we found that overexpression of SH3BP1 promoted the proliferation, migration, and invasion of melanoma cells by increasing Rac1 activity and Wave2 protein levels in vitro. Similarly, overexpression of SH3BP1 facilitated melanoma progression by upregulating Wave2 protein expression in vivo.

Conclusion

In summary, this study revealed for the first time that SH3BP1 promoted melanoma progression through Rac1/Wave2 signaling pathway, providing a new therapeutic target for melanoma.

SUBMITTER: Sun T

PROVIDER: S-EPMC10126683 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

SH3BP1 Regulates Melanoma Progression Through Race1/Wace2 Signaling Pathway.

Sun Ting T Tong Wenxian W Pu Jie J Yu Zhiguo Z Kang Zhengchun Z

Clinical Medicine Insights. Oncology 20230421

<h4>Background</h4>SH3-domain binding protein-1 (SH3BP1), which specifically inactivates Rac1 and its target protein Wave2, has been shown to be an important regulator of cancer metastasis. However, the effects of SH3BP1 in melanoma progression remain unclear. The current study aimed to explore the function of SH3BP1 in melanoma and its possible molecular mechanism.<h4>Methods</h4>TCGA database was used to analyze the expression of SH3BP1 in melanoma. Then, reverse transcription-quantitative pol ...[more]

PMID: 37114076

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Dataset Information

SH3BP1 Regulates Melanoma Progression Through Race1/Wace2 Signaling Pathway.

Background

Methods

Results

Conclusion

Publications

SH3BP1 Regulates Melanoma Progression Through Race1/Wace2 Signaling Pathway.

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