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Data on cloning, expression and biochemical characteristics of a chondroitin sulfate/dermatan sulfate 4-O-endosulfatase.

ABSTRACT: The data shown in this article are related to the published paper entitled "A novel 4-O-endosulfatase with high potential for the structure-function studies of chondroitin sulfate/dermatan sulfate" in Carbohydrate Polymers. In this article, the phylogenetic analysis, cloning, expression, purification, specificity and biochemical characteristics of the identified chondroitin sulfate/dermatan sulfate 4-O-endosulfatase (endoBI4SF) are described in detail. The recombinant endoBI4SF with a molecular mass of 59.13 kDa can can specifically hydrolyze the 4-O- but not 2-O- and 6-O-sulfate groups in the oligo-/polysaccharides of chondroitin sulfate/dermatan sulfate and show the maximum reaction rate in 50 mM Tris-HCl buffer (pH 7.0) at 50°C, which can be a very useful tool for the structural and functional studies of chondroitin sulfate/dermatan sulfate.

SUBMITTER: Wei L

PROVIDER: S-EPMC10126848 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Data on cloning, expression and biochemical characteristics of a chondroitin sulfate/dermatan sulfate 4-O-endosulfatase.

Wei Lin L Xu Yingying Y Du Min M Fan Ying Y Zou Ruyi R Xu Xiangyu X Zhang Qingdong Q Zhang Yu-Zhong YZ Wang Wenshuang W Li Fuchuan F

Data in brief 20230410

The data shown in this article are related to the published paper entitled "A novel 4-O-endosulfatase with high potential for the structure-function studies of chondroitin sulfate/dermatan sulfate" in Carbohydrate Polymers. In this article, the phylogenetic analysis, cloning, expression, purification, specificity and biochemical characteristics of the identified chondroitin sulfate/dermatan sulfate 4-O-endosulfatase (endoBI4SF) are described in detail. The recombinant endoBI ...[more]

PMID: 37113498

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Correction: Chondroitin/dermatan sulfate glycosyltransferase genes are essential for craniofacial development.

Project description:[This corrects the article DOI: 10.1371/journal.pgen.1010067.].

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