Selection and validation of reference genes for the normalization of quantitative real-time PCR in different muscle tissues of rabbits.

ABSTRACT:

Background

In molecular biology studies, the selection of optimal reference genes is of vital importance for accurately quantifying gene expression. The purpose of the present study was to screen the most stable reference genes in different muscle tissues of New Zealand white rabbits and Yufeng yellow rabbits.

Methods and results

Results indicated that the most stable reference genes in the muscle tissues of New Zealand white rabbits were HPRT1, ACTB and PPIC, while HPRT1, PPIC, and RPL13A were the most stable reference genes in muscle tissues of Yufeng yellow rabbits. However, in the longissimus dorsi muscle and the abdominal wall muscle of both varieties, the most stable reference genes were HPRT1, RPL13A, and SDHA. In the quadriceps femoris muscle, the most stable referen

SUBMITTER: Ni M 

PROVIDER: S-EPMC10127086 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature