Lipid-Specific Direct Translocation of the Cell-Penetrating Peptide NAF-1^44-67 across Bilayer Membranes.

ABSTRACT: The cell-penetrating peptide NAF-1 has recently emerged as a promising candidate for selective penetration and destruction of cancer cells. It displays numerous membrane-selective behaviors including cell-specific uptake and organelle-specific degradation. In this work, we explore membrane penetration and translocation of NAF-1 in model lipid bilayer vesicles as a function of lipid identity in zwitterionic phosphatidylcholine lipids mixed with anionic phosphatidylserine, phosphatidylglycerol, and phosphatidic acid lipids. By monitoring the digestion of NAF-1 using the protease trypsin located inside but not outside the vesicles, we determined that the translocation of NAF-1 was significantly enhanced by the presence of phosphatidic acid in the membrane compared to the other three anionic o

SUBMITTER: Drexler CI 

PROVIDER: S-EPMC10127249 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature