A Hitchhiker's Guide to Supplying Enzymatic Reducing Power into Synthetic Cells.

ABSTRACT: The construction from scratch of synthetic cells by assembling molecular building blocks is unquestionably an ambitious goal from a scientific and technological point of view. To realize functional life-like systems, minimal enzymatic modules are required to sustain the processes underlying the out-of-equilibrium thermodynamic status hallmarking life, including the essential supply of energy in the form of electrons. The nicotinamide cofactors NAD(H) and NADP(H) are the main electron carriers fueling reductive redox reactions of the metabolic network of living cells. One way to ensure the continuous availability of reduced nicotinamide cofactors in a synthetic cell is to build a minimal enzymatic module that can oxidize an external electron donor and reduce NAD(P)⁺. In the diver

SUBMITTER: Partipilo M 

PROVIDER: S-EPMC10127272 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature