Mast cells and sphingosine-1-phosphate underlie prelesional remodeling in a mouse model of eczema.

ABSTRACT:

Background

Atopic dermatitis (AD) is a chronic skin inflammation that affects children and adults worldwide, but its pathogenesis remains ill-understood.

Methods

We show that a single application of OVA to mouse skin initiates remodeling and cellular infiltration of the hypodermis measured by a newly developed computer-aided method.

Results

Importantly, we demonstrate that skin mast cell (MC) activation and local sphingosine-1-phosphate (S1P) are significantly augmented after OVA treatment in mice. Deficiency in sphingosine kinase (SphK)1, the S1P-producing enzyme, or in MC, remarkably mitigates all signs of OVA-mediated remodeling and MC activation. Furthermore, skin S1P levels remain unchanged in MC-deficient mice exposed to OVA. LPS-free OVA does not recapitulate a

SUBMITTER: Wedman PA 

PROVIDER: S-EPMC10127444 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature