Imaging and positioning through scattering media with double-helix point spread function engineering.

ABSTRACT:

Significance

Double-helix point spread function (DH-PSF) microscopy has been developed for three-dimensional (3D) localization and imaging at super-resolution but usually in environments with no or weak scattering. To date, super-resolution imaging through turbid media has not been reported.

Aim

We aim to explore the potential of DH-PSF microscopy in the imaging and localization of targets in scattering environments for improved 3D localization accuracy and imaging quality.

Approach

The conventional DH-PSF method was modified to accommodate the scanning strategy combined with a deconvolution algorithm. The localization of a fluorescent microsphere is determined by the center of the corresponding double spot, and the image is reconstructed from the scanned data by deco

SUBMITTER: Gao J 

PROVIDER: S-EPMC10127513 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature