Chromosomal Position of Ribosomal Protein Genes Affects Long-Term Evolution of Vibrio cholerae.

ABSTRACT: It is unclear how gene order within the chromosome influences genome evolution. Bacteria cluster transcription and translation genes close to the replication origin (oriC). In Vibrio cholerae, relocation of s10-spc-α locus (S10), the major locus of ribosomal protein genes, to ectopic genomic positions shows that its relative distance to the oriC correlates to a reduction in growth rate, fitness, and infectivity. To test the long-term impact of this trait, we evolved 12 populations of V. cholerae strains bearing S10 at an oriC-proximal or an oriC-distal location for 1,000 generations. During the first 250 generations, positive selection was the main force driving mutation. After 1,000 generations, we observed more nonadaptative mutations and hypermutator g

SUBMITTER: Larotonda L 

PROVIDER: S-EPMC10127744 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature