Unlocking the strength of inducible promoters in Gram-negative bacteria.

ABSTRACT: Inducible bacterial promoters are ubiquitous biotechnology tools that have a consistent architecture including two key elements: the operator region recognized by the transcriptional regulatory proteins, and the -10 and -35 consensus sequences required to recruit the sigma (σ) 70 subunits of RNA polymerase to initiate transcription. Despite their widespread use, leaky transcription in the OFF state remains a challenge. We have updated the architecture of the lac and tet promoters to improve their strength, control and portability by the adaptation of the consensus -10 and -35 sequence boxes strongly targeted by σ⁷⁰ , incorporation of a strong ribosome binding site recognized broadly by Gram-negative bacteria, and independent control of the transcriptional regulators by constitut

SUBMITTER: Carrillo Rincon AF 

PROVIDER: S-EPMC10128130 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature