Improving growth properties of Corynebacterium glutamicum by implementing an iron-responsive protocatechuate biosynthesis.

ABSTRACT: Corynebacterium glutamicum experiences a transient iron limitation during growth in minimal medium, which can be compensated by the external supplementation of protocatechuic acid (PCA). Although C. glutamicum is genetically equipped to form PCA from the intermediate 3-dehydroshikimate catalysed by 3-dehydroshikimate dehydratase (encoded by qsuB), PCA synthesis is not part of the native iron-responsive regulon. To obtain a strain with improved iron availability even in the absence of the expensive supplement PCA, we re-wired the transcriptional regulation of the qsuB gene and modified PCA biosynthesis and degradation. Therefore, we ushered qsuB expression into the iron-responsive DtxR regulon by replacing the native promoter of the qsuB gene by the promoter P_ripA and introduced

SUBMITTER: Thoma F 

PROVIDER: S-EPMC10128138 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature