Mechanistic Study into Free Radical-Activated Glycan Dissociations through Isotope-Labeled Cellobioses.

ABSTRACT: Inspired by the electron-activated dissociation technique, the most potent tool for glycan characterization, we recently developed free radical reagents for glycan structural elucidation. However, the underlying mechanisms of free radical-induced glycan dissociation remain unclear and, therefore, hinder the rational optimization of the free radical reagents and the interpretation of tandem mass spectra, especially the accurate assignment of the relatively low-abundant but information-rich ions. In this work, we selectively incorporate the ¹³C and/or ¹⁸O isotopes into cellobiose to study the mechanisms for free radical-induced dissociation of glycans. The eight isotope-labeled cellobioses include 1-¹³C, 3-¹³C, 1'-¹³C, 2'-¹³C,

SUBMITTER: Fabijanczuk K 

PROVIDER: S-EPMC10129047 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature