Molecular mechanism of tRNA binding by the Escherichia coli N7 guanosine methyltransferase TrmB.

ABSTRACT: Among the large and diverse collection of tRNA modifications, 7-methylguanosine (m⁷G) is frequently found in the tRNA variable loop at position 46. This modification is introduced by the TrmB enzyme, which is conserved in bacteria and eukaryotes. However, the molecular determinants and the mechanism for tRNA recognition by TrmB are not well understood. Complementing the report of various phenotypes for different organisms lacking TrmB homologs, we report here hydrogen peroxide sensitivity for the Escherichia coli ΔtrmB knockout strain. To gain insight into the molecular mechanism of tRNA binding by E. coli TrmB in real time, we developed a new assay based on introducing a 4-thiouridine modification at position 8 of in vitro transcribed tRNA^Phe enabling us to fluoresce

SUBMITTER: Schultz SK 

PROVIDER: S-EPMC10130221 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature