Protocol for bacterial typing using Fourier transform infrared spectroscopy.

ABSTRACT: The Fourier transform infrared (FT-IR) signals obtained from bacterial samples are specific and reproducible, making FT-IR an efficient tool for bacterial typing at a subspecies level. However, the typing accuracy could be affected by many factors, including sample preparation and spectral acquisition. Here, we present a unified protocol for bacterial typing based on FT-IR spectroscopy. We describe sample preparation from bacterial culture and FT-IR spectrum collection. We then detail FT-IR spectrum preprocessing and multivariate analysis of spectral data for bacterial typing.

SUBMITTER: Yang H 

PROVIDER: S-EPMC10130498 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature