Validation of novel forensic DNA markers using multiplex microhaplotype sequencing.

ABSTRACT: Microhaplotypes (MH) are comprised of multiple single nucleotide polymorphisms (SNPs) that are located within 300 bases of genomic sequence. Improved tools are needed to facilitate broader application of microhaplotypes in a diverse range of populations and forensic settings. We designed an assay for multiplex sequencing of 90 microhaplotypes (mMHseq) that include 46 MH loci with high Effective Number of Alleles (A_e) from previous studies [1], and 44 high A_e MH loci containing between four to fourteen SNPs that were identified from the 1000 Genomes (1KG) Project. The unique design of mMHseq integrates a novel method for multiplex amplification from small DNA amounts, and multiplex sequencing of 48 samples in a single MiSeq run to detect all relevant MH variation. Assa

SUBMITTER: Gandotra N 

PROVIDER: S-EPMC10131188 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature