Sperm-borne microRNA-34c regulates maternal mRNA degradation and preimplantation embryonic development in mice.

ABSTRACT:

Background

Studies have shown that sperm-borne microRNAs (miRNAs) are involved in mammalian preimplantation embryonic development. In humans, spermatozoan miR-34c levels are correlated with in vitro fertilization outcomes, such as embryo quality and the clinical pregnancy and live birth rates. In rabbits and cows, miR-34c improves the developmental competence of embryos generated by somatic cell nuclear transfer. However, the mechanisms underlying the regulation of embryonic development by miR-34c remain unknown.

Methods

Female C57BL/6 mice (6-8 weeks old) were superovulated, and pronucleated zygotes were collected and microinjected with an miR-34c inhibitor or a negative-control RNA. The embryonic development of the microinjected zygotes was evaluated, and the messenger RNA

SUBMITTER: Cui L 

PROVIDER: S-EPMC10131327 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature