Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands.

ABSTRACT:

Background

Wolbachia pipientis is an endosymbiont bacterium that induces cytoplasmic incompatibility and inhibits arboviral replication in mosquitoes. This study aimed to assess Wolbachia prevalence and genetic diversity in different mosquito species from Cape Verde.

Methods

Mosquitoes were collected on six islands of Cape Verde and identified to species using morphological keys and PCR-based assays. Wolbachia was detected by amplifying a fragment of the surface protein gene (wsp). Multilocus sequence typing (MLST) was performed with five housekeeping genes (coxA, gatB, ftsZ, hcpA, and fbpA) and the wsp hypervariable region (HVR) for strain identification. Identification of wPip groups (wPip-I to wPip-V) was performed using PCR-restriction fragment length polymorphism (RFLP)

SUBMITTER: da Moura AJF 

PROVIDER: S-EPMC10131387 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature