Dynamic ctDNA Mutational Complexity in Patients with Melanoma Receiving Immunotherapy.

ABSTRACT:

Background

Circulating tumour DNA (ctDNA) analysis promises to improve the clinical care of people with cancer, address health inequities and guide translational research. This observational cohort study used ctDNA to follow 29 patients with advanced-stage cutaneous melanoma through multiple cycles of immunotherapy.

Method

A melanoma-specific ctDNA next-generation sequencing (NGS) panel, droplet digital polymerase chain reaction (ddPCR) and mass spectrometry analysis were used to identify ctDNA mutations in longitudinal blood plasma samples from Aotearoa New Zealand (NZ) patients receiving immunotherapy for melanoma. These technologies were used in conjunction to identify the breadth and complexity of tumour genomic information that ctDNA analysis can reliably report.

Res

SUBMITTER: Fitzgerald S 

PROVIDER: S-EPMC10131510 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature