From defaults to databases: parameter and database choice dramatically impact the performance of metagenomic taxonomic classification tools.

ABSTRACT: In metagenomic analyses of microbiomes, one of the first steps is usually the taxonomic classification of reads by comparison to a database of previously taxonomically classified genomes. While different studies comparing metagenomic taxonomic classification methods have determined that different tools are 'best', there are two tools that have been used the most to-date: Kraken (k-mer-based classification against a user-constructed database) and MetaPhlAn (classification by alignment to clade-specific marker genes), the latest versions of which are Kraken2 and MetaPhlAn 3, respectively. We found large discrepancies in both the proportion of reads that were classified as well as the number of species that were identified when we used both Kraken2 and MetaPhlAn 3 to classify reads wit

SUBMITTER: Wright RJ 

PROVIDER: S-EPMC10132073 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature