Distinct stage-specific transcriptional states of B cells derived from human tonsillar tissue.

ABSTRACT: B cells within secondary lymphoid tissues encompass a diversity of activation states and multiple maturation processes that reflect antigen recognition and transition through the germinal center (GC) reaction, in which mature B cells differentiate into memory and antibody-secreting cells (ASCs). Here, utilizing single-cell RNA-seq, we identify a range of distinct activation and maturation states of tonsil-derived B cells. In particular, we identify what we believe is a previously uncharacterized CCL4/CCL3 chemokine-expressing B cell population with an expression pattern consistent with B cell receptor/CD40 activation. Furthermore, we present a computational method that leverages regulatory network inference and pseudotemporal modeling to identify upstream transcription factor modulation al

SUBMITTER: Espinoza DA 

PROVIDER: S-EPMC10132144 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature