The cytochrome b carboxyl terminal region is necessary for mitochondrial complex III assembly.

ABSTRACT: Mitochondrial bc ₁ complex from yeast has 10 subunits, but only cytochrome b (Cytb) subunit is encoded in the mitochondrial genome. Cytb has eight transmembrane helices containing two hemes b for electron transfer. Cbp3 and Cbp6 assist Cytb synthesis, and together with Cbp4 induce Cytb hemylation. Subunits Qcr7/Qcr8 participate in the first steps of assembly, and lack of Qcr7 reduces Cytb synthesis through an assembly-feedback mechanism involving Cbp3/Cbp6. Because Qcr7 resides near the Cytb carboxyl region, we wondered whether this region is important for Cytb synthesis/assembly. Although deletion of the Cytb C-region did not abrogate Cytb synthesis, the assembly-feedback regulation was l

SUBMITTER: Flores-Mireles D 

PROVIDER: S-EPMC10132202 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature