Comparison among plaque assay, tissue culture infectious dose (TCID₅₀) and real-time RT-PCR for SARS-CoV-2 variants quantification.

ABSTRACT:

Background and objectives

SARS-CoV-2 variants of concern (VOC) and interest (VOI) pose a significant threat to public health because the rapid change in the SARS-CoV-2 genome can alter viral phenotypes such as virulence, transmissibility and the ability to evade the host response. Hence, SARS-CoV-2 quantification techniques are essential for timely diagnosis and follow-up. Besides, they are vital to understanding viral pathogenesis, antiviral evaluation, and vaccine development.

Materials and methods

Five isolates of SARS-CoV-2: D614G strain (B.1), three VOC (Alpha, Gamma and Delta), and one VOI (Mu) were used to compare three techniques for viral quantification, plaque assay, median tissue culture infectious dose (TCID₅₀) and real-time RT-PCR.

Results

Plaq

SUBMITTER: Zapata-Cardona MI 

PROVIDER: S-EPMC10132340 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature