In vitro erythrocyte production using human-induced pluripotent stem cells: determining the best hematopoietic stem cell sources.

ABSTRACT:

Background

Blood transfusion is an essential part of medicine. However, many countries have been facing a national blood crisis. To address this ongoing blood shortage issue, there have been efforts to generate red blood cells (RBCs) in vitro, especially from human-induced pluripotent stem cells (hiPSCs). However, the best source of hiPSCs for this purpose is yet to be determined.

Methods

In this study, hiPSCs were established from three different hematopoietic stem cell sources-peripheral blood (PB), cord blood (CB) and bone marrow (BM) aspirates (n = 3 for each source)-using episomal reprogramming vectors and differentiated into functional RBCs. Various time-course studies including immunofluorescence assay, quantitative real-time PCR, flow cytometry, karyotyping, morpholo

SUBMITTER: Cho YK 

PROVIDER: S-EPMC10132444 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature