Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.

ABSTRACT: Recent advances in electronics and microfluidics have enabled several research groups to develop fully integrated, sample-to-result isothermal nucleic acid amplification test (NAAT) platforms for the point of care. However, high component counts and costs have limited translation of these platforms beyond the clinic to low-resource settings-including homes. Many NAATs include complex, multi-component heater electronics based on flex circuits or multiple printed circuit boards (PCBs) to support essential NAAT steps such as lysis, sample deactivation, and nucleic acid amplification. In contrast, current commercial assays for home use, such as those for pregnancy or ovulation that include electronics, typically have just one onboard PCB. This work describes a generalizable strategy to integra

SUBMITTER: Shah KG 

PROVIDER: S-EPMC10132542 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature