MiR-96-5p is involved in alcohol-induced apoptosis in PC12 cells via negatively regulating TAp73.

ABSTRACT:

Objective

The present study opted for the adrenal phaeochromocytoma (PC12) cell line to frame a neuronal injury model induced by alcohol exposure in vitro, aiming to probe whether TAp73 and miR-96-5p are involved in the neuronal injury process induced by alcohol and elucidate the regulatory relationship between miR-96-5p and TAp73.

Methods

Immunofluorescence staining was used to observe the structural features of PC12 cells after culturing in medium with nerve growth factor (NGF). After different doses and different durations of alcohol treatment, CCK-8 assay was performed to detect the viability of PC12 cells, flow cytometry assay was carried out to detect the apoptosis rate of PC12 cells, dual-luciferase reporter assay was used to definitude the regulatory relationship bet

SUBMITTER: Yang B 

PROVIDER: S-EPMC10132643 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature