Potential use of high-resolution melting analyses for SARS-CoV-2 genomic surveillance.

ABSTRACT: The pandemic caused by COVID-19 and the emergence of new variants of SARS-CoV-2 have generated clinical and epidemiological impacts on a global scale. The use of strategies for monitoring viral circulation and identifying mutations in genomic regions involved in host interaction are important measures to mitigate viral dissemination and reduce its likely complications on population health. In this context, the objective of this work was to explore the potential of high-resolution melting (HRM) analysis combined with one-step real-time reverse transcription PCR in a closed-tube system, as a fast and convenient method of screening for SARS-CoV-2 mutations with possible implications on host-pathogen interactions. The HRM analyses allowed the distinction of the Gamma, Zeta, Alpha, Delta, and O

SUBMITTER: de Souza Andrade A 

PROVIDER: S-EPMC10132831 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature