Effects of human placenta cryopreservation on molecular characteristics of placental mesenchymal stromal cells.

ABSTRACT: Cryopreservation of placenta tissue for long-term storage provides the opportunity in the future to isolate mesenchymal stromal cells that could be used for cell therapy and regenerative medicine. Despite being widely used, the established cryopreservation protocols for freezing and thawing still raise concerns about their impact on molecular characteristics, such as epigenetic regulation. In our study, we compared the characteristics of human placental mesenchymal stromal cells (hPMSCs) isolated from fresh (native) and cryopreserved (cryo) placenta tissue. We assessed and compared the characteristics of native and cryo hPMSCs such as morphology, metabolic and differentiation potential, expression of cell surface markers, and transcriptome. No significant changes in immunophenotype and dif

SUBMITTER: Navakauskiene R 

PROVIDER: S-EPMC10133466 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature