Taxonomical composition and functional analysis of biofilms sampled from a nuclear storage pool.

ABSTRACT: Sampling small amounts of biofilm from harsh environments such as the biofilm present on the walls of a radioactive material storage pool offers few analytical options if taxonomic characterization and estimation of the different biomass contributions are the objectives. Although 16S/18S rRNA amplification on extracted DNA and sequencing is the most widely applied method, its reliability in terms of quantitation has been questioned as yields can be species-dependent. Here, we propose a tandem-mass spectrometry proteotyping approach consisting of acquiring peptide data and interpreting then against a generalist database without any a priori. The peptide sequence information is transformed into useful taxonomical information that allows to obtain the different biomass contributions at

SUBMITTER: Pible O 

PROVIDER: S-EPMC10133526 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature