Vaginal Microbiome Metagenome Inference Accuracy: Differential Measurement Error according to Community Composition.

ABSTRACT: Several studies have compared metagenome inference performance in different human body sites; however, none specifically reported on the vaginal microbiome. Findings from other body sites cannot easily be generalized to the vaginal microbiome due to unique features of vaginal microbial ecology, and investigators seeking to use metagenome inference in vaginal microbiome research are "flying blind" with respect to potential bias these methods may introduce into analyses. We compared the performance of PICRUSt2 and Tax4Fun2 using paired 16S rRNA gene amplicon sequencing and whole-metagenome sequencing data from vaginal samples from 72 pregnant individuals enrolled in the Pregnancy, Infection, and Nutrition (PIN) cohort. Participants were selected from those with known birth outcomes and adequ

SUBMITTER: Carter KA 

PROVIDER: S-EPMC10134888 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature