Novel Reversible Inhibitors of Xanthine Oxidase Targeting the Active Site of the Enzyme.

ABSTRACT: Xanthine oxidase (XO) is a flavoprotein catalysing the oxidation of hypoxanthine to xanthine and then to uric acid, while simultaneously producing reactive oxygen species. Altered functions of XO may lead to severe pathological diseases, including gout-causing hyperuricemia and oxidative damage of tissues. These findings prompted research studies aimed at targeting the activity of this crucial enzyme. During the course of a virtual screening study aimed at the discovery of novel inhibitors targeting another oxidoreductase, superoxide dismutase, we identified four compounds with non-purine-like structures, namely ALS-1, -8, -15 and -28, that were capable of causing direct inhibition of XO. The kinetic studies of their inhibition mechanism allowed a definition of

SUBMITTER: Rullo R 

PROVIDER: S-EPMC10135315 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature