Interaction and Redox Chemistry between Iron, Dopamine, and Alpha-Synuclein C-Terminal Peptides.

ABSTRACT: α-Synuclein (αS), dopamine (DA), and iron have a crucial role in the etiology of Parkinson's disease. The present study aims to investigate the interplay between these factors by analyzing the DA/iron interaction and how it is affected by the presence of the C-terminal fragment of αS (Ac-αS_119-132) that represents the iron-binding domain. At high DA:Fe molar ratios, the formation of the [Fe^III(DA)₂]^- complex prevents the interaction with αS peptides, whereas, at lower DA:Fe molar ratios, the peptide is able to compete with one of the two coordinated DA molecules. This interaction is also confirmed by HPLC-MS analysis of the post-translational modifications of the peptide, where oxidized αS is observed through an inner-sphere mechanism. Moreover,

SUBMITTER: Schifano F 

PROVIDER: S-EPMC10135331 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature