Empty and Full AAV Capsid Charge and Hydrophobicity Differences Measured with Single-Particle AFM.

ABSTRACT: Adeno-associated virus (AAV) is showing promise as a therapy for diseases that contain a single-gene deletion or mutation. One major scale-up challenge is the removal of empty or non-gene of interest containing AAV capsids. Analytically, the empty capsids can be separated from full capsids using anion exchange chromatography. However, when scaled up to manufacturing, the minute changes in conductivity are difficult to consistently obtain. To better understand the differences in the empty and full AAV capsids, we have developed a single-particle atomic force microscopy (AFM) method to measure the differences in the charge and hydrophobicity of AAV capsids at the single-particle level. The atomic force microscope tip was functionalized with either a charged or a hydrophobic molecule, and the

SUBMITTER: Heldt CL 

PROVIDER: S-EPMC10135413 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature