Anti-Citrullinated Protein Antibody Reactivity towards Neutrophil-Derived Antigens: Clonal Diversity and Inter-Individual Variation.

ABSTRACT:

Background

Why the adaptive immune system turns against citrullinated antigens in rheumatoid arthritis (RA) and whether anti-citrullinated protein antibodies (ACPAs) contribute to pathogenesis are questions that have triggered intense research, but still are not fully answered. Neutrophils may be crucial in this context, both as sources of citrullinated antigens and also as targets of ACPAs. To better understand how ACPAs and neutrophils contribute to RA, we studied the reactivity of a broad spectrum of RA patient-derived ACPA clones to activated or resting neutrophils, and we also compared neutrophil binding using polyclonal ACPAs from different patients.

Methods

Neutrophils were activated by Ca²⁺ ionophore, PMA, nigericin, zymosan or IL-8, and ACPA binding was s

SUBMITTER: Circiumaru A 

PROVIDER: S-EPMC10135477 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature