Timing of Chromosome DNA Integration throughout the Yeast Cell Cycle.

ABSTRACT: The dynamic mechanism of cell uptake and genomic integration of exogenous linear DNA still has to be completely clarified, especially within each phase of the cell cycle. We present a study of integration events of double-stranded linear DNA molecules harboring at their ends sequence homologies to the host's genome, all throughout the cell cycle of the model organism Saccharomyces cerevisiae, comparing the efficiency of chromosomal integration of two types of DNA cassettes tailored for site-specific integration and bridge-induced translocation. Transformability increases in S phase regardless of the sequence homologies, while the efficiency of chromosomal integration during a specific cycle phase depends upon the genomic targets. Moreover, the frequency of a specific translocation b

SUBMITTER: Tosato V 

PROVIDER: S-EPMC10135612 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature