Force-Regulated Calcium Signaling of Lymphoid Cell RPMI 8226 Mediated by Integrin α₄β₇/MAdCAM-1 in Flow.

ABSTRACT: MAdCAM-1 binds to integrin α₄β₇, which mediates the rolling and arrest of circulating lymphocytes upon the vascular endothelia during lymphocytic homing. The calcium response by adhered lymphocytes is a critical event for lymphocyte activation and subsequent arrest and migration under flow. However, whether the interaction of integrin α₄β₇ /MAdCAM-1 can effectively trigger the calcium response of lymphocytes remains unclear, as well as whether the fluid force affects the calcium response. In this study, we explore the mechanical regulation of integrin α₄β₇-induced calcium signaling under flow. Flou-4 AM was used to examine the calcium response under real-time fluorescence microscopy when cells were firmly adhered to a parall

SUBMITTER: Sun D 

PROVIDER: S-EPMC10135767 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature