Dataset Information

Cryptic mtDNA Diversity of Diopatra cuprea (Onuphidae, Annelida) in the Northwestern Atlantic Ocean.

ABSTRACT: Marine annelid taxonomy is experiencing a period of rapid revision, with many previously "cosmopolitan" species being split into species with more limited geographic ranges. This is exemplified by the Diopatra genus, which has recently witnessed dozens of new species descriptions rooted in genetic analyses. In the northwestern Atlantic, the name D. cuprea (Bosc 1802) has been applied to populations from Cape Cod through the Gulf of Mexico, Central America, and Brazil. Here, we sequenced mitochondrial cytochrome oxidase I (COI) in D. cuprea populations from the Gulf of Mexico to Massachusetts. We find evidence for several deep mitochondrial lineages, suggesting that cryptic diversity is present in the D. cuprea complex from this coastline.

SUBMITTER: Sotka EE

PROVIDER: S-EPMC10136041 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Cryptic mtDNA Diversity of Diopatra cuprea (Onuphidae, Annelida) in the Northwestern Atlantic Ocean.

Sotka Erik E EE Bell Tina T Berke Sarah S

Biology 20230330 4

Marine annelid taxonomy is experiencing a period of rapid revision, with many previously "cosmopolitan" species being split into species with more limited geographic ranges. This is exemplified by the Diopatra genus, which has recently witnessed dozens of new species descriptions rooted in genetic analyses. In the northwestern Atlantic, the name D. cuprea (Bosc 1802) has been applied to populations from Cape Cod through the Gulf of Mexico, Central America, and Brazil. Here, we sequ ...[more]

PMID: 37106722

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Project description:Multiple species in the toxic marine diatom genus Pseudo-nitzschia have been identified in the Northwestern Atlantic region encompassing the Gulf of Maine (GOM), including the Bay of Fundy (BOF). To gain further knowledge of the taxonomic composition and toxicity of species in this region, Pseudo-nitzschia isolates (n=146) were isolated from samples collected during research cruises that provided broad spatial coverage across the GOM and the southern New England shelf, herein referred to as the GOM region, during 2007-2008. Isolates, and cells in field material collected at 38 stations, were identified using electron microscopy (EM). Eight species (P. americana, P. fraudulenta, P. subpacifica, P. heimii, P. pungens, P. seriata, P. delicatissima and P. turgidula), and a novel form, Pseudo-nitzschia sp. GOM, were identified. Species identity was confirmed by sequencing the large subunit of the ribosomal rDNA (28S) and the internal transcribed spacer 2 (ITS2) for six species (36 isolates). Phylogenetic analyses (including neighbor joining, maximum parsimony, and maximum likelihood estimates and ITS2 secondary structure analysis) and morphometric data supported the placement of P. sp. GOM in a novel clade that includes morphologically and genetically similar isolates from Australia and Spain and is genetically most similar to P. pseudodelicatissima and P. cuspidata. Seven species (46 isolates) were grown in nutrient-replete batch culture and aliquots consisting of cells and growth medium were screened by Biosense ASP ELISA to measure total domoic acid (DA) produced (intracellular + extracellular); P. americana and P. heimii were excluded from all toxin analyses as they did not persist in culture long enough for testing. All 46 isolates screened produced DA in culture and total DA varied among species (e.g., 0.04 to 320 ng ml-1 for P. pungens and P. sp. GOM isolates, respectively) and among isolates of the same species (e.g., 0.24 - 320 ng ml-1 for P. sp. GOM). The 15 most toxic isolates corresponded to P. seriata, P. sp. GOM and P. pungens, and fg DA cell-1 was determined for whole cultures (cells and medium) using ELISA and liquid chromatography (LC) with fluorescence detection (FLD); for seven isolates, toxin levels were also estimated using LC - with mass spectrometry and ultraviolet absorbance detection. Pseudo-nitzschia seriata was the most toxic species (up to 3,500 fg cell-1) and was observed in the GOM region during all cruises (i.e., during the months of April, May, June and October). Pseudo-nitzschia sp. GOM, observed only during September and October 2007, was less toxic (19 - 380 fg cell-1) than P. seriata but more toxic than P. pungens var. pungens (0. 4 fg cell-1). Quantitation of DA indicated that concentrations measured by LC and ELISA were positively and significantly correlated; the lower detection limit of the ELISA permitted quantification of toxicity in isolates that were found to be nontoxic with LC methods. The confirmation of at least seven toxic species and the broad spatial and temporal distribution of toxic Pseudo-nitzschia spp. have significant implications for the regional management of nearshore and offshore shellfisheries resources.

Dataset Information

Cryptic mtDNA Diversity of Diopatra cuprea (Onuphidae, Annelida) in the Northwestern Atlantic Ocean.

Publications

Cryptic mtDNA Diversity of <i>Diopatra cuprea</i> (Onuphidae, Annelida) in the Northwestern Atlantic Ocean.

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