Identifying lipid particle sub-types in live Caenorhabditis elegans with two-photon fluorescence lifetime imaging

ABSTRACT: Fat metabolism is an important modifier of aging and longevity in Caenorhabditis elegans. Given the anatomy and hermaphroditic nature of C. elegans, a major challenge is to distinguish fats that serve the energetic needs of the parent from those that are allocated to the progeny. Broadband coherent anti-Stokes Raman scattering (BCARS) microscopy has revealed that the composition and dynamics of lipid particles are heterogeneous both within and between different tissues of this organism. Using BCARS, we have previously succeeded in distinguishing lipid-rich particles that serve as energetic reservoirs of the parent from those that are destined for the progeny. While BCARS microscopy produces high-resolution images with very high information content, it is not yet a widely available platform

SUBMITTER: Chen W 

PROVIDER: S-EPMC10137682 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature